Rapid Detection of Enterohaemorrhagic E.coli
Using Phage-Based Bioluminescent Assay
Published: November 1, 2018 | DOI: https://doi.org/10.7860/JCDR/2018/36748.12251
Sabah AA Jassim, Rand R Hafidh, Zahraa Q Ali, Ahmed S Abdulamir
1. Professor, Department of Environmental Engineering, Applied Bio Research Inc., University of Windsor, Windsor, Canada.
2. Senior Lecturer, Department of Microbiology, College of Medicine, University of Baghdad, Baghdad, Iraq.
3. Senior Lecturer, Department of Anatomy, College of Medicine, University of Baghdad, Baghdad, Iraq.
4. Professor, Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad, Iraq.
Correspondence
Dr. Rand Riadh Hafidh,
Senior Lecturer, Department of Microbiology, College of Medicine, University of Baghdad,
P.O. Box 61023, Postal Code 12114, Baghdad, Iraq.
E-mail: ranria77@yahoo.com
Introduction: There is a need for using a reliable, time-saving, and specific detection assay for coliforms, environmental E. coli, and Enterobacteriaceae worldwide.
Aim: To innovate a new principle of phage-based rapid diagnostic test in detecting E. coli bacteria in low titer and short time.
Materials and Methods: A phage mixture of 200 E. coli specific phages, including 22 specific for Enterohaemorrhagic E.coli (EHEC), were used in a new detection platform, a phage-based Adenylate Kinase Bioluminescence Assay (AKBA). Ten EHEC E. coli and 30 universal E.coli isolates were used for AKBA assay.
Results: AKBA showed positive detection of E.coli bacteria at 1000 CFU/ml in just 20 minutes. The phage-based detection was highly specific at strain level of E.coli. The sensitivity and specificity of AKBA was 74% and 78%, respectively.
Conclusion: A rapid and strain-specific diagnostic test was prepared to E.coli by using coliphages. The significance and impact of the study shows that it might be feasible to formulate a phage-based assay against any Gram negative or positive bacteria using the same approaches of the current AKBA assay with slight modifications.
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